I have accidentally added large amount of plasmid DNA to the OPTI-MEM serum (not mixed with lipofectamine yet) and I want to know if I can keep the mixture to use later?
I want to know more about Uranium ore deposits in world.
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I want to know more about diamond ore deposits in world.
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We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...
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If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?
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I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
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I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...
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How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?
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Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
11 July 2024 4,812 5 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
Hi,I have been trying to transfect MDA MB 231 cell lines since a while, I tried with lipofectamine LTX as well as lipofectamine 3000.Please suggest how to proceed with this cell line and what is...
02 June 2024 1,572 0 View
We have transfected the plasmid with lipofectamine, the plasmid will eventually move into the nucleus for transcription. Does this plasmid remain in the nucleus during cell replication? if so how...
22 May 2024 5,958 2 View
Here's the situation: I am currently using 6-Well plates & HEK293t cells with DMEM +10% FBS + 1% P/S and OptiMEM w/ Lipofectamine 2000 for my transfection. Before transfection when cells are...
22 April 2024 6,153 3 View
Lipofectamine is a commonly used transfection reagent known for its efficiency in delivering nucleic acids into mammalian cells. However, the optimal transfection conditions can vary depending on...
11 April 2024 5,893 2 View
Hello. I've been trying to transfect plasmid DNA to my breast cancer cell lines (BT-474 and SK-BR-3) with Thermo Fisher's Lipofectamine 3000 reagent. According to the thermo fisher, those cells...
24 March 2024 9,489 5 View
I tried several times using the Lipofectamine 2000 reagent, extracted the proteins but couldn't detect on Western blot. My plasmids were constructed with PcDNA 3.1+ Please does anyone have any...
20 March 2024 4,533 4 View
Dear colleagues, I need to transfect a 10 kb plasmid into Caski cells and am hoping to use Lipofectamine 3000 for the transfection. If anyone has experience using Lipofectamine 3000 specifically...
11 January 2024 1,762 1 View
Hello, I am looking for technical advices. I have never worked on mouse cells before. I plan to use lipofectamine RNAiMAX as transfection reagent. Can anyone tell me where can I find protocols or...
08 January 2024 2,680 0 View