Does anyone know good protocol for extraction of nuclear proteome?

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Steven Footitt

Try Eubel et al 2008 Novel Proteins, Putative Membrane Transporters, and an Integrated Metabolic Network Are Revealed by Quantitative Proteomic Analysis of Arabidopsis Cell Culture Peroxisomes. PLANT PHYSIOLOGY 148:1809-1829

DOI:10.1104/pp.108.129999

If you couple nuclei isolation protocol with their protein extraction yo may be in luck.

Elsayed MOHAMED Elazazi

Protein Extraction of Soybean

nuclear proteome extraxtion