Does anyone know any stain kit for labeling the nucleus in an immunocytochemical analysis? Something like NucRed Live 647, but close to 594nm peak excitation. No UV please, we do not have UV in our confocal.
Here is a list of immunofluorescent nuclear stains that work with a range of wavelengths. Especially the yellow Hoescht, which you can use in photoconversion of DAB to obtain an electron-dense precipitate if you want to use immunohistochemistry instead.
Yellow Hoescht https://www.thermofisher.com/order/catalog/product/N21485
List for Molecular Probes (ThermoFisher)
https://www.thermofisher.com/nz/en/home/references/molecular-probes-the-handbook/probes-for-organelles/nuclear-and-chromosome-counterstaining-and-nissl-stains.html#head3
You say immunocytochemical but then give an example of a live cell permeable dye. Are you wanting to stain the nuclei in live or fixed cells?
Is propidium iodide not what you want? It has an emission peak in the low 600s.
If you want to test a range of dyes for fixed cells, you could try this: https://www.thermofisher.com/order/catalog/product/N7565?ICID=search-product
Then maybe methyl green could fit. Please check attached article.
Article A fast, low cost, and highly efficient fluorescent DNA label...
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