Does a parents education level have an impact on their child's education?

Do children follow in the same footsteps as their parents in terms of their education?

12 January 2021 1,375 7 View

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

08 December 2020 2,087 1 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

after dissolving biomass in an ionic liquid and filtering it with denoised water. how may i find out how much of cellulose in my filtrate?

i was wondering if you know of an accurate method for quantifying the amount of cellulose in a filtrate. of biomass that had been dissolved in ionic liquid and then filtered with deionized...

05 November 2020 6,469 2 View

Protocol for fixing mouse livers and fat pads for H&E staining?

Hi there! I need advice on a protocol for mouse tissue staining. I will dissect mouse livers and fat pads and look for liver steatosis and adipocyte perimeter. I was planning on doing this with...

22 November 2019 7,490 6 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

29 April 2019 5,057 3 View

Which statistical test(s) should be run with ordinal data?

The research has: 1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS...

30 November 2018 8,807 4 View

Which statistical test(s) should be run with what kind of data (nominal, ordinal, etc)?

1 IV and 3 DV's. Likert scales used yielding scores of 1-5 on Behavioural Observational Scales (BOS). n=99 with each individual being scored on all 3 DV's. Total BOS=297. 99 BOS scored for each...

29 November 2018 6,231 4 View

What is the best method of alkaloid extraction from insects?

I'm working on a project for which I need to extract the alkaloids in Lycorma Delicatula (the spotted lanternfly) for HPLC, and I'm having a difficult time finding an adequate protocol for...

14 November 2018 9,004 3 View

How can I stain cells in culture supernatant ?

I am doing endothelial cells culture and it seems I have erythrocytes contamination but I want to check that. How can I do it as these cells are only in the supernatant ? Thank you in advance

17 June 2018 1,933 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

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22 February 2021 9,932 11 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View