The company that used to do it closed down so we need to find an alternative. Thanks in advance!
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...
07 July 2024 5,614 5 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
Hi! I have to test a drug on the LSC fraction of THP1, but I'm not finding any specific media to select and culture them. Do you know anyone? Thank you in advance
15 May 2024 1,464 3 View
Hello, I am performing esterification reactions between fatty acids and alcohols, in presence of methanesulfonic acid and H3PO2 as catalysts. At the end of the reaction, to reach the acid index I...
22 April 2024 2,063 5 View
I need to analyze the residual methanol from methyl esters. I am wondering if there is someone who has this method DIN EN 14110 and is willing to share it with me? Thank you in advance, Elena
22 April 2024 387 0 View
Recently my pepsin column got some blockage, supposedly because of the precipitation of aggregated protein. Multiple washes with 0.1% FA or pepsin wash (1.5M GuHCl + AcN + FA 95.2/4/0.8), as well...
20 April 2024 3,135 5 View
I am facing an issue with how chromatograms are recorded. From time to time, on our chromatograms the peak assigned to heptane is integrated as well and this gives us wrong results. Sometimes the...
07 April 2024 1,726 4 View
I need to perform non-parametric post hoc multiple comparisons. I found two interesting macros that implemented methodology about post hoc test for Kruskal Wallis test, but p-value was never...
07 April 2024 288 3 View
Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...
26 March 2024 1,660 11 View
I have noticed that, regardless of whether isolating primary microglia from humans or mice, purity is generally assessed using CD11c flow cytometry staining. Why not use CD11b instead?
02 July 2024 9,411 2 View
I am trying to grow microglia culture for 10 days and after day 2/3 there are certain flat non-microglial contaminating cells observed. They are rapidly growing and some are even growing on top or...
24 June 2024 5,325 2 View
Hello, I am checking the inflammatory reaction that occurs when LPS is treated on BV2 microglia. When I treated LPS on BV2 cells, I confirmed a noticeable increase in IL-6 and I expected that JAK2...
12 June 2024 8,144 3 View
I am working with microglia SIMA9 and I will perform a qPCR. If you have already worked with them, can you suggest me one?
05 June 2024 4,930 3 View
Dear colleagues, I'm planning to start neuron mouse co-culture with astrocyte and microglia but I heard that it's difficult to keep it up. Any advices?
27 May 2024 4,905 3 View
Hello everyone, I am currently growing Human microglia cells HMC3 in 100 mm plate. My question is what is the estimated Protein concentration (within range) of 1 ml HMC3 cell lysate? I usually...
25 April 2024 4,592 3 View
I have two groups that I collected microglia from and did RTqPCR. However, the CT values come up as extremely different (p
15 April 2024 6,268 0 View
I am researching the microglia related neuroinflammation in CNS, and want to know the earliest time of microglia after acute inflammatory stimulation.
26 March 2024 6,209 1 View
I am currently learning to culture primary microglia cells from P0-P2-day-old pups, and after 14 days of culturing, I got these cells. I performed IBA1 staining to check purity, but still, I am...
12 February 2024 8,753 8 View
I would need a human microglia cell line Cirspr-edited KO for TREM2. I am pretty sure to have read a paper with HMC3 trem2 KO but I cannot find it again!! Any help??
05 February 2024 6,117 1 View