The company that used to do it closed down so we need to find an alternative. Thanks in advance!
I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...
24 February 2021 9,210 1 View
16 February 2021 2,857 9 View
Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?
01 January 2021 7,026 2 View
Can anyone please suggest some references? I would like to simulate scattering of a fundamental mode of a fiber by a microsphere. I am only familiar with a plane wave case, but in case of a curved...
01 January 2021 1,440 3 View
I've been trying to extract DNA from rat liver tissue using Blood and tissue DNeasy mini kit. I followed handbook procedure and all of my samples had low 260/230 (
21 December 2020 7,100 3 View
Hi everybody, I am analysing RNA-Seq data of patient samples. One group of samples has a certain monoallelic deletion, the other group 2 copies of the wildtype gene. Now, I want to compare the...
28 November 2020 5,977 3 View
We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse...
16 November 2020 8,701 1 View
I chemically transformed a ligation product (pET28a and my gene of 3051 bp) into E coli DH5alpha competent cells. I plated 200 and 800 ml of my transformation on plates of LB agar Kan (25 mg/ml)....
13 September 2020 4,421 3 View
Hi! I want to distinguish D1R and D2R subpopulations of medium spiny neurons in striatum in immunofluorescence. Most of the papers I found use transgenic EGFP mice targeting this receptors, but I...
31 August 2020 3,863 2 View
Hello, I am looking for the user manual of the geostrain software, I have downloaded the software but the user manual is not found. I will be very grateful for your help Cheers
14 June 2020 4,169 1 View
I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...
11 February 2021 9,509 1 View
Hey everybody, for my project I need to analyze the morphology of microglia, which I have previously stained (IHC) and scanned in a SlideScanner. Specifically, I need the ramification, number and...
31 January 2021 3,855 3 View
Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...
20 December 2020 7,166 2 View
I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...
24 November 2020 2,359 2 View
Dear colleagues, I am studying microglia in early development. I would like to design a study where I deplete microglia in neonatal pups using a CSF-1R inhibitor. Several studies that employ the...
06 November 2020 793 2 View
Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...
14 October 2020 4,572 3 View
I would like to use flow cytometry sorting (Aria III) to sort astrocytes and microglia from adult human and adult mouse brain, with the aim of extracting RNA from sorted bulk pools. Can someone...
11 October 2020 3,624 1 View
Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...
06 October 2020 3,916 3 View
I am a technician at Brigham and Women's Hospital seeking an estimate of the total number of microglia in the mouse retina. So far, I have not been able to find this in the literature. Can anyone...
16 September 2020 5,281 1 View
Antibody based approaches aren't working as well as for other cell types, even IBA1 isn't working that well. Does anyone know of a histological/chromogenic stain for microglia? Or any other way?...
13 September 2020 5,927 3 View