Dear Salman,

please let me know about the study objectives precisely so as to suggest the primers,

GD

Dear Gulba

Thank you because of your attention, Im working on physiology of osmoregulation in mentioned species.

Salman

Dear Salman

first of all you should determine which gene you are going to study

The primers are designed mainly based on the sequences available at NCBI

you can search in NCBI and you will see the sequence of gens available on Catfish

Also you can look in the papers to see the primers that have been already used

Good luck with you research my dear friend

Dear Salman

Good, please mention the Scientific name of the species

It will good, if you could suggest what are you interested in catfish physiology so that we can suggest some relevant information.

Cheers,

GD