I have 5 isolate(wild type, 3mutants and 1 complement) and I want to compare between wild type and them. 

My question is how can I get t.test and p.value? I created each isolate with control (untreated) and 5 treatment.

when I account the survival the number of control and treatment is vary for example wild type control 15 whereas mutant1 =12, mutant2=7.

Do you thing which way can I use to compare? because ANOVA it will compare all of them but still the initial point(control) differ.

Thank you 

Ayat