Does anyone know about klebsiella HSP60 (Groel) antibody?

15 May 2015 1 3K Report

Hi all

I will try to detect hsp60-groel heat shock protein at klebsiella pneumoniae with western blot analysis. But i cant find this antibody. so can i use e.coli groel antibody or common anti groel antibody?

Rakesh Ganji

Hi,

That depends on the cross reactivity of the antibody. Is the epitope used to generate the e. coli (or general groEL) antibody similar to klebsiella protein. If the epitope is same/similar u can give it a try. But, if the antibody is not available commercially, I wud suggest you to generate your own antibody if you have purified protein.

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