Does anyone know a US based company that does sub cloning?

More Gabriela Arias De la Rosa's questions See All

What is the best book for finding characteristics group frequencies in Raman?

I need to make some assignments in my Raman spectra and want to use a book to guide them.

10 November 2019 2,451 3 View

How to batch import the Raman spectrum of several log files in gaussian09?

I have dozens of log files and I will like to know if there is a way to batch import their Raman spectrum files from their log file. I know how to import the Raman spectrum of each file by using...

07 August 2019 4,590 3 View

Is it possible for a protein to bind to a weak cation exchanger at a pH above its pI?

I'm reading about the schemes used to purify a protein from different sources. In one of these schemes, they used buffers with a pH (6.5) above the pI (5.7) of the protein to equilibrate a weak...

03 April 2019 8,818 4 View

pH-effect on the Raman frequency

It is possible that if a group in a bound substrate is exposed to the solvent surrounding the protein complex while the rest of the substrate is buried inside the protein, the pH of the solvent...

06 July 2017 2,955 3 View

Getting the x-intercept instead of the y-intercept in Excel Regression analysis?

Is there any Excel function that gives an output similar to Regression analysis (e.g. Regression statistics, ANOVA, intercept, X variable) but shows the x-intercept instead of the y-intercept?

06 July 2016 4,010 6 View

How can I decrease the fluorescence of my samples for Raman spectroscopy?

I am trying to get the Raman spectra of solutions with very low concentrations of amino acids 1mM-6 mM. However, I can not see their peaks because they are masked by the fluorescence of the...

11 December 2014 610 12 View

Which supplier sells IPTG at a low price?

I need to buy 100 grams of IPTG and the suppliers I have checked charge a small fortune for this amount. Can someone recommend a supplier that sells this reagent at an affordable price?

02 March 2014 7,368 6 View

Has anyone used a Cold-Shock Expression System employing the pCold vector in E. coli?

I am thinking in using this expression system to express dihydropteridine reductase. I want to know if anyone has used this system before and what advantages and disadvantages they have over other...

01 February 2014 1,297 4 View

Can anyone recommend me an ion-exchange column and a gel filtration column?

I need to buy an ion-exchange column and a gel filtration column for a Biologic Duoflow chromatography system. Does anyone knows of any vendors besides Bio-rad that sell these columns? Thanks for...

09 October 2013 10,132 20 View

Could my sonication protocol have degraded my protein?

I sonicated my bacterial suspension on ice, 15 seconds on, 30 seconds off, at 60% amplitude. I repeated this 14 times. After purifying my protein using nickel affinity chromatography, I did a gel...

09 October 2013 7,552 17 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View