What is the best book for finding characteristics group frequencies in Raman?

I need to make some assignments in my Raman spectra and want to use a book to guide them.

10 November 2019 2,290 3 View

How to batch import the Raman spectrum of several log files in gaussian09?

I have dozens of log files and I will like to know if there is a way to batch import their Raman spectrum files from their log file. I know how to import the Raman spectrum of each file by using...

07 August 2019 4,357 3 View

Is it possible for a protein to bind to a weak cation exchanger at a pH above its pI?

I'm reading about the schemes used to purify a protein from different sources. In one of these schemes, they used buffers with a pH (6.5) above the pI (5.7) of the protein to equilibrate a weak...

03 April 2019 8,662 4 View

pH-effect on the Raman frequency

It is possible that if a group in a bound substrate is exposed to the solvent surrounding the protein complex while the rest of the substrate is buried inside the protein, the pH of the solvent...

06 July 2017 2,822 3 View

Getting the x-intercept instead of the y-intercept in Excel Regression analysis?

06 July 2016 3,860 6 View

How can I decrease the fluorescence of my samples for Raman spectroscopy?

11 December 2014 387 12 View

Does anyone know a US based company that does sub cloning?

I need my gene of interest to be subcloned into a bacterial expression vector (pET) to produce a N-terminal His tagged protein cleavable with TEV protease. Also, I want Ampicillin to be the...

01 February 2014 1,619 4 View

Has anyone used a Cold-Shock Expression System employing the pCold vector in E. coli?

I am thinking in using this expression system to express dihydropteridine reductase. I want to know if anyone has used this system before and what advantages and disadvantages they have over other...

01 February 2014 1,146 4 View

Can anyone recommend me an ion-exchange column and a gel filtration column?

I need to buy an ion-exchange column and a gel filtration column for a Biologic Duoflow chromatography system. Does anyone knows of any vendors besides Bio-rad that sell these columns? Thanks for...

09 October 2013 9,982 20 View

Could my sonication protocol have degraded my protein?

I sonicated my bacterial suspension on ice, 15 seconds on, 30 seconds off, at 60% amplitude. I repeated this 14 times. After purifying my protein using nickel affinity chromatography, I did a gel...

09 October 2013 7,390 17 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View