14 Questions 19 Answers 0 Followers
Questions related from Gabriela Arias De la Rosa
I need to make some assignments in my Raman spectra and want to use a book to guide them.
11 November 2019 2,417 3 View
I have dozens of log files and I will like to know if there is a way to batch import their Raman spectrum files from their log file. I know how to import the Raman spectrum of each file by using...
08 August 2019 4,531 3 View
I'm reading about the schemes used to purify a protein from different sources. In one of these schemes, they used buffers with a pH (6.5) above the pI (5.7) of the protein to equilibrate a weak...
04 April 2019 8,775 4 View
It is possible that if a group in a bound substrate is exposed to the solvent surrounding the protein complex while the rest of the substrate is buried inside the protein, the pH of the solvent...
07 July 2017 2,918 3 View
Is there any Excel function that gives an output similar to Regression analysis (e.g. Regression statistics, ANOVA, intercept, X variable) but shows the x-intercept instead of the y-intercept?
07 July 2016 3,978 6 View
I am trying to get the Raman spectra of solutions with very low concentrations of amino acids 1mM-6 mM. However, I can not see their peaks because they are masked by the fluorescence of the...
12 December 2014 576 12 View
I need to buy 100 grams of IPTG and the suppliers I have checked charge a small fortune for this amount. Can someone recommend a supplier that sells this reagent at an affordable price?
03 March 2014 7,332 6 View
I need my gene of interest to be subcloned into a bacterial expression vector (pET) to produce a N-terminal His tagged protein cleavable with TEV protease. Also, I want Ampicillin to be the...
02 February 2014 1,722 4 View
I am thinking in using this expression system to express dihydropteridine reductase. I want to know if anyone has used this system before and what advantages and disadvantages they have over other...
02 February 2014 1,264 4 View
I sonicated my bacterial suspension on ice, 15 seconds on, 30 seconds off, at 60% amplitude. I repeated this 14 times. After purifying my protein using nickel affinity chromatography, I did a gel...
10 October 2013 7,510 17 View
I want to know if imidazole concentrations of up to 250 mM or 1% triton x-100 can interfere with this assay.
10 October 2013 485 2 View
I need to buy an ion-exchange column and a gel filtration column for a Biologic Duoflow chromatography system. Does anyone knows of any vendors besides Bio-rad that sell these columns? Thanks for...
10 October 2013 10,100 20 View
I have purified His tagged proteins in the past, but I am a bit confused on how to purify doubly tagged proteins. I tried looking in the literature on how to do this, and I have not found anything...
06 June 2013 8,255 5 View
I need to determine the protonation states the complexes form by citrate and Zn(II) have at different pHs.
01 January 2012 1,528 10 View
