I am looking for a protocol that allow to reproducibly perform LTT to diagnose idiosyncratic drug reactions with SOPs to define drug concentrations and time of incubation (and if possible, cut-offs)
These are here on RG:
Lymphocyte transformation test: a new method for diagnosis of neurocysticercosis
- June 2008
- Diagnostic Microbiology and Infectious Disease 61(2):198-202
- 📷Amit Prasad
- 📷Kashi Nath Prasad
- 📷Abhishek Yadav
- Show all 8 authors
- Mazhar Husain
See an article we wrote in the 1960's: Coulson and Chalmers, 1966, Quantitation of Peripheral Blood Lymphocyte Cultures, Nature, 209, 378. This method involves physically counting the actual number of small lymphocytes that have transformed; yielding an index of response.
This addresses the time factor (90 hours of culture), the viabilty of the cells, and the 'background" spontaneous transformation of cells.
Manually counting the cells is the 'best' way in my opinion, but is time consuming. So many researchers use indirect methods such as DNA synthesis.
Thank you for this helpful answer. Are you aware of any SOPs for the test that are widely accepted?
No, can't help with SOP question.
But it occurred to me that most techniques allow the cells to settle to
the bottom of the culture vessel, which means that one is measuring
the response of the entire cultured population.
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