Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

More Harpreet Kaur Ranjit Singh's questions See All

How to carry out flow cytometry on whole blood?

I want to observe the different cell populations (mainly leukocytes) from whole blood. Are there any recommended protocols that I could follow?

11 December 2019 1,131 4 View

How much blood would I need to culture T cells in a T-75 flask?

I will be isolating T cells from blood samples and culturing them in a T-75 flask. What would be the required blood volume needed? Or rather, how much blood would I need to have enough of cells to...

06 July 2019 1,532 1 View

Does HSP27 show up in endogenous levels for HEK293 and A549 cells or do they only show up under stress?

Was wondering if hsp27 shows up only during stress or also in endogenous levels

01 February 2018 1,173 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Michael Förster

Dear Harpreet Kaur Ranjit Singh

please see the link below from Miltenyi Biotec, which nicely lays out defining markers for eosinophils and basophils, isolation protocols from PBMC and flow cytometry plots, which should help your gating strategy.

https://www.miltenyibiotec.com/CA-en/resources/macs-handbook/human-cells-and-organs/human-cell-types/granulocytes-human.html

All the best & kind regards,

Michael

Harpreet Kaur Ranjit Singh

Michael Förster this is really helpful. Thank you very much!