Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

More Harpreet Kaur Ranjit Singh's questions See All

How to carry out flow cytometry on whole blood?

I want to observe the different cell populations (mainly leukocytes) from whole blood. Are there any recommended protocols that I could follow?

11 December 2019 1,028 4 View

How much blood would I need to culture T cells in a T-75 flask?

I will be isolating T cells from blood samples and culturing them in a T-75 flask. What would be the required blood volume needed? Or rather, how much blood would I need to have enough of cells to...

06 July 2019 1,459 1 View

Does HSP27 show up in endogenous levels for HEK293 and A549 cells or do they only show up under stress?

Was wondering if hsp27 shows up only during stress or also in endogenous levels

01 February 2018 1,102 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Michael Förster

Dear Harpreet Kaur Ranjit Singh

please see the link below from Miltenyi Biotec, which nicely lays out defining markers for eosinophils and basophils, isolation protocols from PBMC and flow cytometry plots, which should help your gating strategy.

https://www.miltenyibiotec.com/CA-en/resources/macs-handbook/human-cells-and-organs/human-cell-types/granulocytes-human.html

All the best & kind regards,

Michael