Which plant may act or help for the liquefaction of phlegm or biofilms?

Infections with several germs, which are building a biofilm, are often insensitive against antibiotics. I am looking for herbs, which may be able to disintegrate biofilms like ACC.

08 September 2013 6,037 7 View

Can Calea zacatechichi solve sleeping disorders?

Calea zacatechichi induce dreaming and have a relaxing effect. Is there any knowledge about the influence of serotonin action?

04 May 2013 3,966 6 View

Resistant starches of the type R3 and recommendations of suppliers to get some probes?

I want to test out the effect of these starches in dried herb crackers.

04 May 2013 4,567 1 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Where can I find a database of antibiotics-dose required to kill bacteria?

Hi, I am Zaizhi. I would like to know where I can find a database of antibiotics-dose required to kill different bacteria. I found a few years ago. There, I was able to know the range of dose of...

08 November 2020 9,276 3 View