Does cleaning up cDNA after reverse transcription reduce late cycle background?

I've been wondering if removing the random primers from the reverse transcription reaction either with a column or precipitation before using it as a qPCR template reduces the background a bit in...

25 December 2020 6,258 4 View

Are you a virtual team member/leader who would like to participate in a Delphi study on International Online Collaboration Competencies (IOCC)?

You are invited to participate as expert panel member in our consensus study on International Online Collaboration Competencies (IOCC). The purpose of this study is to build consensus on the key...

11 November 2020 9,715 1 View

Bonferroni or Tukey: Which post-hoc test is adequate for my data?

Hello, I am running an ANOVA between 3 groups (Group 1 N=148, Group 2 N=147, Group 3- N=144; equal variances assumption met) . I am having difficulties in deciding upon the most adequate post-hoc...

21 October 2020 2,182 5 View

What are reaction conditions for aromatic sulfonation of a dibenzyl ether derivative?

I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...

04 August 2020 4,446 7 View

Can you recommend any publications or authors on ethical and eco-friendly marketplaces?

Investigating the difference in business strategies between ethical marketplaces and traditional online marketplaces such as amazon

27 July 2020 7,302 3 View

What are your nanoindentation tips cleaning tricks?

Dear users of nanoindenters, I was wondering what procedures do you usually use to clean your diamond nanoindentation tips? I know that there is nothing specifically described but some internal...

08 July 2020 5,926 5 View

How good is the quality of spectra taken by portable NMR spectrometer?

At the moment our laboratory would like to buy portable NMR spectrometer and we would like to see some real not promo spectrograms. Could those who constantly work with such devices share spectra...

22 June 2020 8,144 6 View

What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method?

What areas of the brain can be measured using EEG? What functions are appropriate and not appropriate to study with this method? Can the EEG record accurately activity in deeper brain structures...

11 March 2020 1,087 6 View

Online testing solutions: any online tool permitting recording of participants' verbal responses?

Covid-19, apart from all health consequences, is also having an impact on research projects with human participants. Universities and schools are shutting down and, even when labs are still open,...

11 March 2020 2,711 16 View

How should I analyse my luciferase data?

02 March 2020 6,149 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View