Calculation the Pump power needed in a pressurized flow system?

Hi all, I am design now a liquid high pressure flow system to be used up to 50 bar, and I am having some doubts concerning how I can estimate the pump power. Besides the pressure drop in the...

15 December 2020 8,411 1 View

Design of a membrane system to remove CO2 from a flue gas current?

Hi all, I am trying to design a membrane system to remove the CO2 from a flue gas current, and I am having difficulties in finding information. Does anyone have any technical information,...

15 December 2020 3,655 2 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

What pressures should I choose in a reheated rankine cycle?

09 October 2020 9,117 4 View

I want to measure cytokine levels in mouse spinal cord tissue and I can not decide which method is best, a cytokine array or ELISA?

Hi everyone. So I need to measure different cytokines in mouse spinal cord tissue and I can not decide which method is the best one, a cytokine array or ELISA. Does anyone that has experience in...

26 July 2020 1,757 2 View

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Nutritional science has provided functional foods with the required amounts of natural antioxidants, vitamins, fats, proteins, polyphenols, carbohydrates, and other components required to maintain...

03 June 2020 7,377 1 View

Is there any protocol capable of simulating an epileptic seizure in vitro using direct electric stimulation?

I am trying to simulate epileptic seizure activity in cell cultures by using direct electric stimulation. However, I am struglling to find an acessible and relatively easy-to-use protocol. Thank...

28 January 2020 4,951 2 View

How can I map brain in live rats after a certain stimulus without an imaging technique?

I need to look for brain rat regions activation after a certain stimulus made to live animals. The marker to be looking for needs to be maintained fro long periods because the stimulus that I need...

10 December 2019 8,626 2 View

How to avoid non-negative solutions in ODE solvers in R?

I'm using the package deSolve in R to model a biological ODE system, however I have the problem of, with certain parameters or with certain initial conditions, the ode solver produces negative...

09 December 2018 7,114 6 View

Are advances in Anti-Aging Research Therapy to reverse cell death critical to Global Anti-Aging Markets expected to EXCEED $331 billion by 2021?

RELEVANT REFERENCES: 1. Martins IJ. Advances in Aging and Health Research. Scientific Research Publishing, Inc. ISBN: 978-1-61896-569-1 2. Martins IJ. Scientific Nutritional Health and Global...

11 September 2018 2,602 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View