Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

DoE: Possible reasons for having a lot of significant effects? Is it as complex or went something wrong?

I did a product development using Design Expert for planning and evaluation. There is a response with a lot of significant effects shown in the Half Normal Plot. By testing factors A, B, C, D I...

06 December 2020 5,128 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Controlling for a variable that is an outcome of the dependent variable: what is the interpretation of the coefficient of the regressor?

I am estimating a dependent variable as a function of a regressor using Ordinary Least Squares regression. If I add a control variable that is (partly) an outcome of my dependent variable, how do...

06 October 2020 3,224 1 View

Which EEG source analysis plugin for Matlab do you recommend?

Good morning, I have the EEG recordings of 65 subjects and I would like to reconstruct the source of the signal in the gamma frequency range. Unfortunately, I don't have an individual MRI or...

25 August 2020 10,021 2 View

How can I study the clusterization Vglut1 puncta in cultured cells ? Do you know exactly the dimension of one single Vglut puncta?

Hi guys, I ave to study the Vglut1 puncta density/clusterization in cultured cells and see any differences between 2 different conditions. I noticed that in treatment B Vglut puncta appear...

29 March 2020 6,120 1 View

Botany - Betula pubescens old drawing?

Does any of you have a vintage botanical print of Betula pubescens (alba) subsp. celtiberica? Thanks a lot!!!

27 February 2020 6,049 5 View

Why do I obtain wavy bands on acrylammide precast gel in western blot experiments?

Hello! I recently tested a new precast gradiented gel for my Western Blot experiments and I obtained wavy bands both for proteins and for molecular weight marker. I run in tris-glycine-sds running...

28 July 2019 2,303 6 View

Tris HCl 1.5M pH 8.8, Tris HCl 1 M pH 6.8, how to pH?

I am experiencing many problems in obtaining the correct pH of Tris-HCl solutions for acrylammide gels (pH8.8 and 6.8) due to the dependence of the pH on temperature. I prepared and brought the...

18 June 2019 7,663 9 View

Does anyone has any references for scholarly articles on teacher professional development and teacher identity?

My field research was to study the interactions of teacher leaders in a teacher leader PLC in a primary school. I am curious as to any findings you'd be willing to share and any current scholarly...

30 December 2018 8,201 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View