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Does anyone have tips for expanding a vector from a supplier? It's ~5ng/uL and stored in 50% glycerol. I attempted to do a transformation as normal but I did not get any growth.
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I need advice on how to maintain the water bath in my cell culture incubator. What materials or methods should I use? I have to use Aquaguard 1, but I see that it has been discontinued. Any advice...
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