I'm running a study in which I will inject some nanoparticles into mice and then section the lymph node and visualize it with confocal. Does anyone have the protocal for the lymph node sectioning and staining? Thanks.
Hi Fan,
Sectioning and staining of lymph node is not different from other tissues.
But you know that thickness of lymph node compare to other tissue. Therefore, you have to make very thin slice of tissue. If you already inject dye labeled nanoparticles then you can just use nuclear stain and visualize under confocal microscope.
You can section out the tissue whatever thin size you want using microtome machine provided if you are expertise in handling it or can take help of expertise.
Hi Vincent,
Are you working with sentinel lymph nodes (with biopsy, nano particles and machine-based imagery) or axillary lymph nodes (with dissection and immuno-histological evaluation)?
In the case of the former, dendrimers seem to be a good option (with some qualifications):
www.ncbi.nlm.nih.gov/pmc/articles/PMC6309687/
www.ncbi.nlm.nih.gov/pmc/articles/PMC3164439/
https://academic.oup.com/jnci/article/96/9/703/2521712 (detailed methodology)
I hope this helps :)