Vincent Fan

4 Questions 7 Answers 0 Followers

Questions related from Vincent Fan

Optimal Does of Nanoparticles for Injection?

I have synthesized some nanoparticles functionalized with antigen and I want to inject them into mice to image their bio-distribution. What are the optimal amount and concentration of the...

01 January 2020 9,278 3 View

Optimal Conditions for DBCO-azide Reaction?

I was trying to conjugate dye and PEG onto DBCO functionalized nanoparticles. The dye I use is azide-Cy5 and the PEG is azido-PEG24-NHS. I first react the dye with particles for 1 whole day...

10 October 2019 5,217 3 View

Does anyone have the protocol for lymph node sectioning and imaging?

I'm running a study in which I will inject some nanoparticles into mice and then section the lymph node and visualize it with confocal. Does anyone have the protocal for the lymph node sectioning...

09 September 2019 4,876 3 View

Incubation Time for Primary Antibody Staining?

Hello, I wanna stain some RAW 264.7 macrophages with anti-mice MHC-II and CD80 antibodies to run flow cytometry. Last time I tried 4C for 1hr but it barely worked. This time I'm thinking maybe I...

12 December 2018 6,289 13 View