I have synthesized some nanoparticles functionalized with antigen and I want to inject them into mice to image their bio-distribution. What are the optimal amount and concentration of the nanoparticle suspension to be injected into the mice? Thanks.
The amount of toxic substance in the content of the synthesized nanoparticle is important. Because it can prevent blood clotting. The toxic picture can take shape quickly. Therefore, you must report the content of the synthesized substance.
The dosage and concentration can depend on a lot of factors like the route of injection (i.p, i.m, oral, etc), the buffer in which you want to dissolve.