I am cloning endolysins and need phage phiKZ. I will gladly pay shipping costs. Please email me at [email protected] if you have this phage and will share it. Thank you!
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...
08 July 2024 4,887 2 View
Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...
19 June 2024 4,420 3 View
I am trying to reconstitute the bacterium B.ovatus ATCC. it was first placed in a THIO broth and despite this we observed no turbidity. This was done 24h after the stick containing B.ovatus ATCC...
12 June 2024 1,644 3 View
How can you regulate the number of random inserted mutations in error prone PCR?
14 May 2024 673 2 View
Good evening, My IPA (Interpretative Phenomenological Analysis) research project involves conducting interviews in a different country and in a different language. Considering that I will need to...
05 May 2024 6,147 2 View
I am using this code (below) to use FDR to correct p values because I ran three exploratory growth curve models. How many p values do you need to correct? Do I just correct all of the p values for...
02 May 2024 4,028 4 View
We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!
05 April 2024 6,269 4 View
Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...
04 April 2024 4,896 1 View
Can I use feces preserved in formaldehyde to perform the McMaster technique for Haemonchus research?
20 March 2024 1,339 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View