Please do add a picture of how it is seen under normal microscope.
we have been trying to standardize the procedure for a while and have not been succesful.
Dear Sreejith Nair,
from your personal profile I found you're MSc - Student - School of Science, GU; Zoology Department ( unfortunately lacking your city and country?) so I do not know about the possibility of your guaranteed and free access to Google searching database or at least Google.Scholar or PubMed.
I would like to ask honestly - also for further viewers of your question - for your including in this thread as a short reply or by editing your question about the method of AO-staining you tried to use and to standardize (you wrote: "...picture of how it is seen under normal microscope.... guessing you therefore use bright field microscopy but not sure about, because an other possibility is/would be fluorescence microscopy and / or the use e. g. of Giemsa stain instead of AO.).
You only want to observe [only the morphology of the ] nuclei (in "binucleated" PBL-cells)... or viability/non-viability of cells too?
There are some (different) recipes for that task and maybe there is some trick to get optimal and reliable results.
Best of luck W.M.
Dear Mr. Wolfgang H. Muss,
Thank you for the reply. we intend to observe it under the fluorescence microscope too however since the lab with the facility is not the place where we work we wanted to know how it looks under normal brightfield microscope so that we can be sure that we have managed to standardize the staining procedure and are able to get the cells stained.
We only wish to observe the binucleated cells.
We tried staining with A.O however we are not able to get very good results with the same.
I am in India in Ahmedabad city in Gujarat.
Hope you would be able to guide us.
Regards,
Nair Sreejith
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