We are looking for a collaboration on this strain in order to complete our studies.
Hi everyone, I would like to ask for the scientific community's help about colony PCR for generation confirmation. I have isolated bacteria from mice faeces and, after a first characterisation...
24 March 2024 6,347 2 View
On pregnancy day 18 we sacrificed one pregnant rat and we isolated the Hipothalami of the embryos (there were approximately 15 pups). All the Hypothalami were collected in one test tube. They were...
17 February 2022 8,171 3 View
Hello, We are looking for rating ou scoring scales (from 0 to 9) to evaluate blooming intensity and vegetative development of plants (tomatos and ornamental plants) in researches. Thanks !
13 April 2021 4,729 2 View
I collected this skeleton shrimp from some algae and have not been able to match it to typical mediterranean endemic and invasive species. Have considered: Caprella acanthifera, C. dilatata, C....
19 January 2021 4,319 3 View
I'm using generalized linear mixed models (GLMMs) with the package nlme (Pinheiro et al., 2018), employing a Gaussian distribution. My study only has 6 samples with 72 replicas (Petri dishes)....
18 January 2021 2,375 2 View
how can i stop following this project?
18 July 2020 7,584 2 View
I am trying to scale a reservoir fluid flow of 1-foot length per day in the reservoir to simulate the needed coreflood flow rate I should use in the lab. I have noticed that many coreflooding...
23 March 2020 4,399 6 View
Hello, I'm wondering if anyone else has used methoxamine hydrochloride in pyridine as a derivitization agent for GC-MS, and knows relatively how long it can be used before it needs to be remade....
12 November 2019 9,611 1 View
I'm researching about the effect of temperature on legume production so I want to know if there are specific genes that legumes have that help the withstand different temperatures
09 June 2016 4,422 4 View
for example, having a score of 130 on either the verbal comprehension or the perceptual organization ?
15 December 2015 7,171 11 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View