Dear colleagues, could you tell me if it is possible to use Trypan Blue staining for E. coli, in order to differentiate dead cells from the live ones? I have used this technique only for eukaryotic cells, but I am not sure that it can be used in the case of bacteria. If it is possible, could you provide me the protocol? Thank you in advance!
it could be also called as exclusion staining since trypan blue stains only the dead cells and not the live cells. probably it is well suited to stain E.coli with this stain with same procedueres adopted for procaryotic staining 0.4% trypan blue stain is used
One method that I have used in some of my previous experiments it to couple trypan blue with a bacterial expressed fluorescent protein (such as GFP). This approach operates by inducing expression of the fluorescent protein and applying your treatment. At time-points that you'd like to measure viability, simply incubate trypan blue with the cells for a short period of time (
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