Does anyone have knowledge on the disruption of kidney tissue with a bed mill homogenizer?

01 October 2014 1 7K Report

Hi, I need to do RNA and protein extraction of many kidney samples that are frozen at -80ºC in AllProtect reagent. I would like to use a multiple sample homogenizer like Tissuelyser LT, Bullet blender or Mini-Beadbeater 16 to avoid cross contamination using a IKA polytron.

Do you have any experience with these sort of homogenizers? Which one would you recommend me? My budget is not very high.

I am going to do WB and Real-time PCR with these samples.

Thank you

Elena Guillén

Thank you Seweryn! Qiagen buys steel beads (5 and 7 mm) and tungsten carbide (3mm) beads . Otherwise I use kidney tissue that is relatively easy to homogenize, so tissuelyser LT could be a good choice.

Thank you for your help!

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