Elena Guillén

5 Questions 14 Answers 0 Followers

Questions related from Elena Guillén

Primary monocyte cultures, cryopreserve or not?

Hello, everybody, I'm interested in culturing monocytes isolated from human peripheral blood. I would like to know if it is as correct to use cryopreserved monocytes or if it is better not to...

07 June 2019 688 16 View

Can room temperature affect Western-blot?

Hello, everyone, I've been doing Western-blocks for years. For a few days now I have had problems, even with "easy" antibodies such as GAPDH. According to my findings, proteins are in good...

01 June 2018 9,160 3 View

Is CD14 a good marker for THP-1 treated with PMA?

Hello, everybody, I need to treat THP-1 cells after differentiating them with PMA. I have read many articles and there is not much consensus on the conditions (seeding density, concentration of...

23 October 2017 6,214 2 View

Does anyone have knowledge on the disruption of kidney tissue with a bed mill homogenizer?

Hi, I need to do RNA and protein extraction of many kidney samples that are frozen at -80ºC in AllProtect reagent. I would like to use a multiple sample homogenizer like Tissuelyser LT, Bullet...

01 October 2014 7,185 1 View

Molecular weight marker for non-reduced proteins

I'm doing some western blots with a large protein (180 kDa) under non-reduced conditions. The band I observed in the western is about 220kDa. Does anyone know if the molecular weights from my...

20 November 2012 8,997 11 View