Dear Colleagues,

Several colorimetric methods were used to quantify the principal components (carbohydrates, proteins, lipids) without any indications on their limits of detection. I want to have information supported by references on the limits of dectection of the following methods:

-Proteins according to Bradford (1976),

-Lipids following Goldsworthy et al. (1972),

-Carbohydrates (Duchateau and Florkin, 1959).

References:

Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities on protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254

Duchateau G, Florkin M (1959) Sur la tréhalosémie des insectes et sa signification. Arch Int  Physiol Biochem 67(2): 306–314.

Goldsworthy GJ, Mordue W, Guthkelch J (1972). Studies on insect adipokinetic hormones. General and Comparative Endocrinology 18: 545–551.

Thanks