What is the reference solution for bacteria community analysis by using qPCR?

Dear fellows, Does anyone know how to make the reference/control solution for bacteria community analysis by using qPCR? My sample is treated water.Thanks

01 February 2015 8,288 1 View

Could I estimate biovolume by using phase-contrast microscope?

Dear Folks, Mostly journals stated that I need Uthermohl, inverted microscope, or other sophisticated tools to estimate biovolume of certain algae species. But here, I only have phase-contrast...

31 December 2014 2,629 1 View

Does anyone have a list of bacteria found in treated water?

31 December 2014 5,771 2 View

What blanko solution and mobile phase can I use for determining the amount of kanamycin and amoxicillin by HPLC?

Dear Fellows, I would like to conduct HLPC to analyze the amount of antibiotics (kanamycin and amoxicillin) from the water. Can anyone suggest to me the common blanko solution and mobile phase for...

09 October 2014 5,195 3 View

What is the relationship between pregnancy and olfactory response?

06 July 2014 483 5 View

Is 24 hours long enough for insect acclimatization?

Normally, the duration of acclimatization for all animals (including insects) is one week. But, I found a journal explained that only needs 24 hour and then insect could be treated. Do you have...

06 July 2014 3,466 7 View

Any suggestions on biopesticide research using binary choices?

06 July 2014 8,195 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View

How to perform Cox proportional hazard analysis or Kaplan-meier survival analysis in SPSS?

At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...

15 February 2021 9,145 2 View