Does anyone have experience with Western Blot for phospho-tau in human brain?

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Lidia Sabater

Do you have a positive control for phosphorylation, An alzheimer patient brain, for instance?.

Do you have run in parallel a total tau antibody also?

Dick Terwel

Aggregared pTau is Alzheimer brains can readily be measured with CP13 or AT8. However, normal Tau becomes quickly dephospphorylated post-mortem and cannot be measured.

Damir Janigro

I am not so sure, we did a study published a few month ago where we detected P-tau in post-mortem and freshly isolated brain (see PMID: 26556772). But thanks so much for your response, Dick.

Damir