We are trying to run experiments with the Biolog EcoPlates to characterize microbial communities from nose swabs and other body sites. After incubation (both with and without shaking) we notice color formation in some of the wells, however the color is concentrated on the side of the well, rendering the measurement inaccurate. We have also noticed before the incubation that the substrate seems to adhere to the side of the wells in the plate, and pipetting does not help. Support from Biolog did not notice anything strange with this particular batch of plates we are using, so I am wondering if I am missing some basic technique here? Has anyone had similar issues?
Dear Valeria Agamennone
These articles might be helpful, have a look:
1. https://res.mdpi.com/d_attachment/applsci/applsci-09-04035/article_deploy/applsci-09-04035.pdf
2. https://res.mdpi.com/d_attachment/nanomaterials/nanomaterials-11-01777/article_deploy/nanomaterials-11-01777.pdf
3. https://www.preprints.org/manuscript/201911.0130/v1/download
Kind Regards
Qamar Ul Islam