I'm using Malme3 cells to analyse cell death with the help of flow cytometry (using annexine and PI). However it seems these cells are difficult to analyse as the dot plot gives a long straight diagonal population where I can't distinguish the different population of living and dead cells.... Has anyone used these cells before? Did you have to optimize the parameters on the flow cytometer? Are they perhaps autofluorescent?
Hye Antonio, thank you for your answer and the paper!
I already use malme 3M cells in my experiments and analysing them with flow cytometry was pretty easy. However the malme 3 fibroblasts (derived from the same person) are really tricky to analyse (plus they grow very slow). Within a few weeks I'll analyse them again and hopefully I can get some results...
- Badges
- Science topic
- Similar topics
- Geoscience
- Cartography
- Mapping
- Maps