first: I had extracted a natural polysaccharide polymer, how can I know it is (1,4) or (1,6).
second: I had prepared complexes with this polysaccharide and transition metal ions, How can I know the chelating sites of this polysaccharide.
xps analysis my be help to you for examine of chelating sites
If it is soluble in a suitable solvent (e.g. D2O) proton NMR might help with the glycosidic linkage question. If the transition metal affects nuclear relaxation rates, NMR might also help with the complexation question.
Multi dimensional NMR, I suggest 3D NMR (HOC) of starting polymer and metal-polymer complex.
Hi, the most simple way is to use edited HSQC spectrum. If 1,6- linkage is present in your polysaccharide you should see H6,H6´/C6 cross peaks shifted to cca 70ppm for carbon. Otherwise, if you need detailed structural features of your polymer, this question can be answered (after a complete assignment of protons for each sugar unit) by NOESY, ROESY or HMBC experiment.
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