To assay Neurospora culture for phosphatase assay, by p-DPP method??
As I am going on with my proteomic approach. Now, I am stuck at one step I want to look inside the nucleus protein expression profile of different tissues. I am finding some of the kits available...
01 February 2015 1,208 2 View
I am following one protocol by that I am trying to take out nucleus proteins but one of my buffer having SDS and in this case I can not use the same sample for 2DE analysis. I tried another...
01 February 2015 814 2 View
I am looking for a method of forming single cell suspension from tumor and normal tissue. I went through some of the discussion (I got 2 only) and I found they have used 1% trypsin for 16 hour at...
01 February 2015 8,569 4 View
I want to check for the different expression levels of p53 protein and I am doing this for the first time. I know about the UV mediated stress for p53 stabilization. So I just want to know if...
02 March 2014 2,181 4 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
