Amit Gupta

5 Questions 6 Answers 0 Followers

Questions related from Amit Gupta

Anyone familiar with the formation of single cell suspension for tumor and normal tissue as well?

I am looking for a method of forming single cell suspension from tumor and normal tissue. I went through some of the discussion (I got 2 only) and I found they have used 1% trypsin for 16 hour at...

02 February 2015 8,554 4 View

How do I isolate nuclei from cancer and normal tissue?

As I am going on with my proteomic approach. Now, I am stuck at one step I want to look inside the nucleus protein expression profile of different tissues. I am finding some of the kits available...

02 February 2015 1,190 2 View

How can I remove SDS from my protein lysate for 2DE analysis?

I am following one protocol by that I am trying to take out nucleus proteins but one of my buffer having SDS and in this case I can not use the same sample for 2DE analysis. I tried another...

02 February 2015 795 2 View

Does anyone have experience with Phosphatase assays by p-DPP method?

To assay Neurospora culture for phosphatase assay, by p-DPP method??

08 August 2014 7,777 0 View

Does anyone know how to induce stabilization of human tumor protein 53?

I want to check for the different expression levels of p53 protein and I am doing this for the first time. I know about the UV mediated stress for p53 stabilization. So I just want to know if...

03 March 2014 2,168 4 View