I think if you treat this 1% SDS Tris buffer as water it seems ok and it will work in PCR.

Dear....,

you need not filter the homogenate to limit host tissue....Even in your normal method, you need not go for this filtration. Because, PCR by design and theory amplifies only the specific and targeted DNA irrespective of the presence of other DNA populations unless you are using degenerate primers or heavy concentration of DNA.

All the best for your research....

@Binech C.P,

I am more than sure many will argue that "PCR .... amplifies only the specific and targeted DNA irrespective of the presence of other DNA ". Be that truth, there would be no need to design protocols like touch down PCR - another approach that I hopefully gonna use to solve my problem

Dear Yuri,

PCR should be very specific as Binesh told you, even so, if your problem is co-amplification of target and host I think you should consider sequencial centrifugation using a sugar gradient or even percoll gradient of the homogenate, if you know the density of spores it should be easy to get the perfect gradient and get almost a perfect enrichment of your target DNA (only spores), after enrichment you can proceed with standard DNA extraction.

Good Luck

Luis