Luis Cunha

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Questions related from Luis Cunha

Filtering for contamination when assembling a genome, before or after assemby?

We are assembling a genome of an non model organism that it s highly dependent of its symbionts. Although, the genomic DNA was extracted from a "less contaminated" body part we still could get...

08 August 2013 2,862 8 View

Correcting SOLID mate-pair reads using high quality reads (for De Novo assembly)?

I have 3 mate-pair SOLID libraries (different sizes) that I would like to use in a De Novo genome assembly, but I found that random error is incredible high and unexpected among the reads,...

08 August 2013 5,097 16 View

What would be the best way to assess diversity between and within populations of a species with uncertain ploidy level using AFLPs?

The fact that polyploidy has consequences for the population structure and diversity, as the same rate of migration of individuals between populations will lead to a lower degree of genetic...

08 August 2013 8,947 3 View