What could be source of contamination for seeing positive amplification in negative control?

Yesterday, I ran a gradient endpoint PCR with some primers and saw positive amplification for positive and negative for negative amplification. Running the same assay today, show positive...

13 January 2021 5,201 1 View

Has anybody found better anticancer effect on patient derived primary cancer cells than cell line?

I am testing compounds on the patient's primary cancer cells as well as a cell line. Generally, I see better activity in cell lines compared to primary cells. Surprisingly I am seeing the...

18 March 2020 467 3 View

Catalyst and electrode for fuel cell application?

Hello, could anybody help me to get idea about which kind of ionomers, surfactants, additives and solvents except Nafion, iso-propanol, n-propanol, hexanol, methanol, ethanol, water, ePTFE,...

11 November 2018 8,886 7 View

How to Calculate the density of ZnO/Graphene Based Composite?

I have prepared ZnO and Graphene based nanocomposites. For the synthesis of this nanocomposite i have used ZnCl2 as precursor for ZnO. For a fixed volume fraction 0.015 vol%, i have to calculate...

20 January 2018 6,269 1 View

How to Calculate the density of ZnO and Graphene Based Nanocomposite?

I have prepared ZnO and Graphene based nanocomposites. For the synthesis of this nanocomposite i have used ZnCl2 as precursor for ZnO. For a fixed volume fraction 0.015 vol%, i have to calculate...

20 January 2018 714 3 View

Regarding K Means Clustering Technique?

Hello Experts, Can we use K Means Clustering for predicting Software Maintainability at design level?

30 March 2016 7,230 4 View

Regarding Software Maintainability ?

Sir, I have taken 22 different UML Class diagram( samples) of 2 different variables and first one contains 4 independent variables and second one contains 7 independent variables.In this situation...

10 February 2016 1,372 2 View

How to find out understandability, analysability and modifiability?

08 February 2016 2,605 1 View

How to isolate terpenes and sesquiterpenes from methnolic extract

How to isolate terpenes and sesquiterpenes from methnolic extract

05 December 2012 1,528 11 View

What is the usual concentration of Diaporthe fungus using DNeasy mini kit protocol?

Hello Everyone, I am using DNeasy mini protocol to extract DNA from at least 7 days old culture in PDA. When I tried it, several times I am getting less than 10 ng/micro liter of DNA. It is...

01 January 1970 2,960 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View