Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Online miRNA target prediction tool and related- down stream pathways?

There are many miRNA target prediction softwares, can you help me from your experience which one is the best? What are the consideratios I must take? because I have no experience in it...

02 September 2020 6,714 5 View

Fault analysis with Simulink- abnormal response after fault clearance?

The attached simple model with SIMULINK after clearing the fault, the Simulink is having abnormal responses. Bus voltage V3 is oscillating. anyone facing that issue?

09 April 2020 2,882 3 View

Continuous IV, DV, M and categorical control variable in mediation. How do you proceed?

Hi everyone! I ask you how you would proceed in the case in which, for a mediation analysis, all considered variables (X, M, Y) are continuous while a control variable to test is categorical (not...

15 January 2019 5,073 3 View

Can I appeal against a journal editorial decision?

Can I appeal against a journal editorial decision?

28 November 2018 8,076 50 View

How to convert Ti (integral time) and Td (derivative time) to I and D parameter in function block parameter for PID controller (SIMULINK)?

I need help on my problem. I managed to tune my PID controller using Ziegler-Nichols continuous cycling method and able to determine my Kp, Ti (integral time) and Td (derivative time). But the...

22 May 2017 5,641 8 View

What is the difference between generalised additive model and polynomial regression?

13 June 2016 6,758 5 View

How can I put cluster labels in the Gaussian Mixture Model (GMM)?

I used GMM to cluster my data. It has 4 dimensions with 3 clusters. The GMM ran very well, and I got the mean and covariance matrix for each cluster. The problem is I don't know which label...

15 October 2014 7,213 5 View

Where can I find researchers interested in education reform and health problems affecting students caused by problems in education?

I'm extremely interested in issues concerning students' heath in educational settings such as stress levels in class rooms and how standardized testing affects drug use on campuses. I would like...

27 July 2013 7,421 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

Hi, Can anyone please tell me what are the ranges of four different pole orders such as first order, second order, third order and fourth order respectively of butterworth filter for ECG...

23 February 2021 2,554 3 View

How to perform Cox proportional hazard analysis or Kaplan-meier survival analysis in SPSS?

At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...

15 February 2021 9,145 2 View

How do you interpret theoretical vs. experimental reaction orders with respect to a reactant?

I recently came across a paper focusing on the hydrodeoxygenation of oxygen-containing compounds. The paper linearized the rate equation in order to determine the experimental reaction order with...

14 February 2021 8,254 2 View

Hello .Ineed a researchs about systolic heart failure any one can help me ?

Am a master student .I need an information about this case to write my thesis?

09 February 2021 9,706 2 View

ECG and children with autism ?

Does anyone have experience with collecting ECG data on children with autism? How was your experience? I'm interested in the tolerance levels for kids to have wires attached to their chests. What...

02 February 2021 5,850 3 View

Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

Is there any method I can use to isolate single cells (i.e cardiomyocytes and non-cardiomyocytes) from snap from rat heart tissue?

I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...

19 January 2021 4,044 3 View

How to specify flow rate boundary condition to these bars in COMSOL?

Hi, I am working on topology optimization. Now, I want to minimize the pressure drop in the pink domain (Fig1). I also need to control the flow by using flow equality (Fig2). I have tried to cut...

01 January 2021 2,833 3 View

What is the best method for removing power-line interference (PLI)?

In the paper A wavelet-based method for power-line interference removal i... we propose a new method for removing PLI. This method consists in identifying the ECG and noise frequency range for...

29 December 2020 9,233 3 View