Can someone help me to how to optimize the chromatin digestion for my CHIP experiment?

Hi everybody, I am doing CHIP experiment. The problem is that in my gel agarose I see alot of DNA with big size (longer that 1000 bp) which means there is alot of unfragmented DNA in my gel. I...

06 September 2020 1,507 5 View

Can someone explane the difference between spheroids generated from primary cells and spheroid produced from cell line?

Hello everyone, I see a lot of papers with the spheroid generation topic. I know spheroids are used widely for simulation of tumor condition in body. Can someone say which abilities does the...

31 August 2020 3,323 3 View

Does anybody know the difference of the effect of the dextran coated charcoal stripped serum (DCSS) containing medium and DCSS containg medium?

Hi everybody, I have cultivated the C4-2 cell line in double charcoal stripped serum containing medium. A lot of the cells do not attach to the bottom of the flask. If someone has experience...

24 August 2020 9,634 2 View

Does someone have experience with cultivation of C4-2 cells in 15 cm dishes?

I am culturing C4-2 cells in 15 cm dishes in different serumes of 5% FCS and 5% DCSS. since the cells are slow in attaching to the dish in DCSS compare to the FCS if someone has experience, can...

20 August 2020 1,535 1 View

Can someone explain how we can Ioptimize antibody concentration which I wanna use in CHIP-qpcr?

Hi everybody, I wanna use an antibody to determine the presence of a protein in my chip-seq experiment. Can someone help me how can do concentration optimization for antibody which I use ? Thanks...

19 July 2020 9,619 3 View

What will the future of sustainable production and consumption within non-durable goods industry?

Assuming that non-durable goods last less than three years on average, which direction do you think the world businesses and consumers are moving considering the huge environmental impact of...

27 May 2020 4,062 4 View

What are the most influential factors that can move world businesses towards practising environmental sustainability?

The industrial world is confronting the environmental side of sustainability. But, how do you evaluate the incentives - either from society or government- to convince them about sustainability...

09 May 2020 557 47 View

Has someone experience about getting different lifetime result by changing magnification of a sample?

Hi everybody, Does anybody have experiences like getting different lifetime result by changing magnification from a sample? Can this factor lonely has influence on the lifetime of a tissue? Bests

20 March 2020 8,745 3 View

Can someone suggest a sterilized, clean and trustable pig serum company for ordering?

Hi everyone, I currently did some experiments with a pig serum (notnheat inactivated), which the control negative of that showed bacterial contamination. As I have to filter it befor using and I...

01 December 2019 8,649 3 View

How much is the possible maximum dose for cells without showing toxic effect?

I extract the primary RPE cells from pig. As I see in several time extraction high contamination of bacterial in my cell culture flask in the 3rd day after extraction. Sometimes they survive...

18 November 2019 8,623 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Thermal reduction of graphene oxide.?

I am trying to reduce graphene oxide by thermally but I have no proper information regarding this. Can any one help me regarding the details about temperature and time for the reduction of...

23 February 2021 4,155 4 View