I am trying to measure nitric oxide levels produced by alveolar epithelial cells following exposure to cytokines. I have only carried out griess assay and believe dan is much more efficient at detecting NO levels.
Perhaps Dan may be assayed by diazotization and coupling. Then estimation can be done by spectrophotometry. There may be the possibility of diazotization of amine group on naphthalene. So you have to try by that. Dan may be soluble in acid like hydrochloric acid.