Thanks
Hi, I am currently using Iba1 for detecting activated microglia. Does anyone know any antibodies that are specific for M2 activation? Thanks.
11 December 2014 7,990 3 View
I am injecting 30mg/kg minocycline after dissolving and slightly heating to 50oC. Do I need to check pH? Is anyone else injecting mino?
08 September 2014 9,053 0 View
Does anyone have a good protocol for paraffin embedding adult rat brains? I don't have a tissue processor. But I can do it manually. thanks
07 August 2014 9,783 7 View
Juvenile rats or adults. Need to look at plasticity changes. Personal experience helps.
10 November 2013 543 2 View
I am writing my phd proposal for neuropharmacology.
08 September 2013 4,354 6 View
I am dissolving at 30 mg/ml in distilled H20, but falls out of solution. Anyone have a better protocol for keeping it in solution?
07 August 2013 1,619 8 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
