Juvenile rats or adults. Need to look at plasticity changes. Personal experience helps.
-LONG-
Dear Partha,
just to add another source (other sources for convenience and your information):
Dariusz Orlowski , and Carsten R. Bjarkam
Histochem Cell Biol (2009) 132:369–374 (contact me if you need the article)
DOI 10.1007/s00418-009-0611-3; @ http://www.ncbi.nlm.nih.gov/pubmed/19504264
Abstract cf. == http://link.springer.com/article/10.1007%2Fs00418-009-0611-3
Stain & Staining -Procedure (briefly as of the article in Section: Materials & Methods):
< Golgi-Cox staining >
The Golgi-Cox solution was prepared from 3 stock solutions named A, B and C, accordingly to [ Glaser and Van der Loos, 1981 ] :
[[Note: this reference not cited in the original article:
Glaser EM., van der Loos H (1981) Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. in: J Neurosci Methods 4: 117-125. This reference taken from:
,
by Emma Perez-Costas, Miguel Melendez-Ferro, and Rosalinda C. Roberts in:
Modern Research and Educational Topics in Microscopy.
A. Méndez-Vilas and J. Díaz (Eds.) pp.164-170, 2007 to be found
cf. @ http://www.formatex.org/microscopy3/pdf/pp164-170.pdf ]]
A is a 5% solution of potassium dichromate in distilled water.
B is a 5% solution of mercuric chloride in distilled water.
C is a 5% solution of potassium chromate in distilled water.
100 ml A was mixed with 100 ml B.
80 ml C was mixed with 200 ml distilled water.
Solution AB was mixed with dilution C under continuous stirring with a glass rod. The obtained solution was stored in a glass bottle in the dark for at least 5 days before use. A precipitate would now form at the bottom and top of the solution. Only fluid free of precipitate was used for tissue immersion in the dark at room temperature. After 5, 10, 20 or 40 days of immersion in the Golgi-Cox solution, tissue samples were washed in distilled water for 1 min followed by ammonium hydroxide (28% solution diluted 1:1 with distilled water immediately before use) for 30 min (Gibb and Kolb 1998).
< Autometallographic enhancement >
The AMG developer was prepared according to Danscher (1981) (using Gum arabic) (contact Gorm Danscher via ResearchGate for a .pdf)
Note added: AMG without gum arabic can be done also (as per article/reference):
< Silver development in microscopy and bioanalysis: a new versatile formulation for modern needs >. by GEOFFREY R. NEWMAN and BHARAT JASANI, in
Histochemical Journal 30, 635±645 (1998)
cf.Pubmed: http://www.ncbi.nlm.nih.gov/pubmed/9870764
(or contact me for a .pdf)
Good luck, best wishes, Wolfgang
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