What statistical test should I use for the comparison of proportion between groups, one of the proportion being 0?

I would like to compare the proportion of mice with resistant bacteria in their faeces. I have three groups, and in one of them not a single mouse has resistant bacteria. I cannot perform Chi2...

25 August 2020 3,579 2 View

How do you deal with large ANSYS Workbench analyses?

Hello, I am trying to evaluate stresses on a model which is quite large and I have memory limitations. I've seen cases ( http://www.padtinc.com/blog/submodeling_ansys_mechanical/ ) where people...

10 July 2020 5,913 3 View

Lamp (bulb) for iCycler iA iQ5 bio-rad..?

Hello everyone, Has anyone ever change the bulb (lamp) in a iCycler iA iQ5 bio-rad? Bio-rad does not sell this lamp anymore, and I'm searching for an alternative solution. However a bulb with...

27 November 2019 2,467 1 View

I was wondering if there are qualitative articles about what leads to impulse buying?

07 October 2019 850 5 View

I'm looking for a PK software that can simulate time-concentration graphs via inputting various variables (ie. Vd, kel, F, etc.)?

Thanks in advance! Purpose is for teaching.

19 August 2019 6,293 1 View

Can any one help me with an analysis grid of school books including indicators on the development of logical reasoning ?

Can any one help me with an analysis grid of school books including indicators on the development of logical reasoning in mathematics, experimental sciences ?

11 June 2019 3,525 4 View

How to blast a fast file with multiple sequences in a genome?

Hello to all, I have a complete genome of a plant and a fast file with multiple sequences of a specific protein ( nucleotide sequence). I want to blast the file into the genome to see if these...

08 February 2019 3,473 5 View

Formvar-coated grids - grey colour, best method?

I need to prepare formvar-coated grids for TEM. I usually use an uncommon method of a drop of formvar on water, however the formvar film is usually uneven. Another and more common method is by...

03 December 2018 2,250 5 View

Long term storage of Cholera Toxin B ?

Hi, I am planning to use Cholera Toxin B for neuroanatomical tracing. The CTB conjugated with Alexa Fluor dyes can be obtained in the solid (500 ug or 100 ug powder) form from suppliers and,...

05 November 2017 8,721 1 View

Hippurate hydrolysis test for Campylobacter jejuni negative after long term storage at -70?

Hippurate hydrolysis test was positive for Campylobacter jejuni ATCC 33560 and for other C. jejuni isolated from stool and other human samples. After 6 month storage at -70 degree hippurrate...

16 May 2017 4,535 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View