PCR used to work, now it doesn't, why?

I recently did a PCR with certain primers, and the reaction went well for two gels, but when I repeated the exact same reaction a third time it suddenly didnt work, then I did it a 4th time and it...

12 December 2020 364 5 View

How to model bubble in Ansys Fluent?

I am seeking to model a simple bubble growth/collapse in Ansys. I have tried using 2D, VOF with an initial patch air circle geometry surrounded by water. I include surface tension and make the air...

10 December 2020 9,113 3 View

DNA disappears from gel, whats going on?

Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it...

28 October 2020 4,427 4 View

How to cite chinese names?

This may look like an odd question, but since I'm latinamerican I'm not familiar with chinese names, the usual way to reference names is by writting the last name and the initial of the names, but...

11 September 2019 9,386 3 View

ANOVA with 4 factors to contrast seasonal curves?

28 April 2019 5,115 1 View

ANOVA with 4 factors to contrast seasonal curves?

03 April 2019 4,284 1 View

Does anyone knows this plant?

I need samples of a plant named Kermadecia sinuata, according to the data I got it is found in New Caledonia, anyone knows any repository of exotic species that could have this specimen and that...

12 February 2019 2,125 2 View

How to separate very closed PCR bands?

I've been trying to purifiy my electrophoresis bands but they are very closed to each other, I've tried 2% agarose gels, ran at 90V for 2 hours and this is as far as I can get them to separate. My...

07 November 2018 5,383 3 View

Somebody has isolated DNA with basic phenol using the DNA/Protein that is precipitate using the protocol of RNAzol or Trizol?

I perform total RNA isolation according to RNAzol RT protocol from bone marrow samples, but I need the DNA of this samples too. Some protocols explain that I can use DNAzol to isolate the DNA but...

03 April 2018 3,979 4 View

Is there any research about traditional cheese-pressing?

We are analysing the traditional ways of cheese-pressing in the Basque Country. There are many materials about cheese and cheese-making, but, unfortunately, we have not found anything about...

26 February 2018 5,870 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View