Omar Sepúlveda-Robles

3 Questions 6 Answers 0 Followers

Questions related from Omar Sepúlveda-Robles

Somebody has isolated DNA with basic phenol using the DNA/Protein that is precipitate using the protocol of RNAzol or Trizol?

I perform total RNA isolation according to RNAzol RT protocol from bone marrow samples, but I need the DNA of this samples too. Some protocols explain that I can use DNAzol to isolate the DNA but...

04 April 2018 4,022 4 View

Could anyone help me to interpret a T. cruzi staining with a cytotoxicity Kit staining in flowcitometry?

In the plot (Calcein vs Etidium), generally, I can see the dead cells in the upper-left position (cells killed using heat 56°/20 min) and the live cells in the down-right position. However, when I...

05 May 2015 1,997 2 View

Does anyone have experience or know if it is possible to use the PFGE technique (SpeI enzyme) for typing eukaryotic organisms like T. cruzi?

Thanks in advance for your replies.

01 January 2015 3,012 4 View