Does anyone have experience of overexpressing recombinant glypican 3 in E. coli? I am having trouble to get it done even after changing its codons to favor expression in E coli.
I was performing quenching studies in solution state. While plotting the Stern-Volmer graph, which intensity should I use as initial intensity (I0). For example, I take 1 ml of 0.1 M fluorophore...
25 June 2024 5,385 1 View
Expecting a technical reason
19 June 2024 4,495 0 View
My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After several months of storage in a refrigerator...
18 June 2024 7,459 5 View
My liposome is a non-phospholipid liposome (sterosome) composed of palmitic acid and cholesterol, 20mg of palmitic acid and 70mg of cholesterol. After a month of storage in a refrigerator at 4...
18 June 2024 1,858 1 View
It doesn't seem like nanowire, because the Tio2 surface is clearly visible.
27 May 2024 6,319 0 View
I am searching for a technical reason that anyone could suggest please?
08 May 2024 6,032 2 View
After staining and solidifying my agarose gel, I load the first well of the dried agarose gel with the TrackIt Ladder (10488058) from Invitrogen and load my unstained DNA samples into the other...
22 April 2024 635 4 View
Hello fellow scholars, I would like to know if I measure the drug release of my liposome through a dialysis membrane, how to solve the model that this device may cause [two-stage release]? Of...
09 April 2024 370 0 View
Tengo una columna de la variable de "precipitación" donde predominan los ceros y hay valores altos. No he podido transformarla para obtener una distribución normal, ya he usado métodos de log,...
05 April 2024 8,427 1 View
I have a fluorophore and its emission is quenched on adding analyte compound. The overlapping spectra of compound and analyte make UV-vis absorption analysis unreliable. The analyte mixture...
03 April 2024 7,914 1 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View