I am trying to observe 2 protein complexes on supported lipid bilayer using AFM. Protein 1 is his-tagged and attached to the lipid bilayer (on glass) wth a Tris-NTA head. Protein 2 is the binding protein that can be in solution. I am not interested in dynamics through AFM, only static pictures of these protein complexes on the bilayers. The complex is formed by the dimer of protein 1 and possibly a dimer of protein 2. I am looking for some guidance on the AFM settings: air vs solution AFM, probe type, peak force (voltage) etc. The microscope in question is the Bruker Bioscope Resolve. Thank you for any inputs!