Does anyone have any suggestions on a comet assay for DNA damage and chemoresistance testing?

More Melissa Chua's questions See All

Can we derive organoids from 2D cell culture?

I am keen to derive organoids from my existing primary tumour cell culture. Is there any existing protocol that you would recommend?

02 March 2018 2,564 3 View

Why does shRNA-mediated silencing have different effects on cell signalling as compared to that by siRNA-mediated silencing?

I started off by creating a stable knockdown model using a control shRNA and two shRNA specific for my gene-of-interest. I achieved 90% knockdown after antibiotic selection, and the knockdown...

07 August 2014 7,765 7 View

Can anyone help with loading controls for high molecular weight proteins?

My proteins of interests are ~350-500kDa. The usual loading controls have much lower molecular weights. I run 6% (non-gradient) gels. Are there any suitable loading controls out there?

01 February 2014 1,434 5 View

Is vinculin an ideal loading control for large proteins?

I recently started using vinculin as a loading control for high molecular weight proteins. Strangely, vinculin showed up evenly despite a 2-fold difference in loading. I wonder what is happening...

01 February 2014 8,671 3 View

Does lysing cells for too long harm phosphoproteins?

I lysed my cells in 1x Laemmli buffer for 2 hours by mistake (the usual time period is 15mins). Would this mistake degrade my samples? I'm looking for phosphoproteins.

10 November 2013 4,945 3 View

If protein of interest is at undetectable mRNA level, is knockdown data still valid?

A protein of interest has an undetectable mRNA expression level in my cell line. I knockdown using siRNA (and overexpressed, but that's a different story) and surprisingly was able to detect the...

06 July 2013 972 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Petra Smatlikova

NEUTRAL comet assay will detect DSBs, whereas ALKALINE comet assay will detect SSBs and DSBs (and the majority of abasic sites as well as alkali labile DNA adducts).

you can read something about it here

http://www.trevigen.com/docs/protocol_4250-050-K.pdf

Albino Bacolla

You can also check the method in this paper: Oncotarget. 2014 Jan 30;5(2):351-62. ERG induces a mesenchymal-like state associated with chemoresistance in leukemia cells. Mochmann LH1, Neumann M, von der Heide EK, Nowak V, Kühl AA, Ortiz-Tanchez J, Bock J, Hofmann WK, Baldus CD.