Does anyone have any suggestions on a comet assay for DNA damage and chemoresistance testing?

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Petra Smatlikova

NEUTRAL comet assay will detect DSBs, whereas ALKALINE comet assay will detect SSBs and DSBs (and the majority of abasic sites as well as alkali labile DNA adducts).

Albino Bacolla

You can also check the method in this paper: Oncotarget. 2014 Jan 30;5(2):351-62. ERG induces a mesenchymal-like state associated with chemoresistance in leukemia cells. Mochmann LH1, Neumann M, von der Heide EK, Nowak V, Kühl AA, Ortiz-Tanchez J, Bock J, Hofmann WK, Baldus CD.