7 Questions 2 Answers 0 Followers
Questions related from Melissa Chua
I am keen to derive organoids from my existing primary tumour cell culture. Is there any existing protocol that you would recommend?
03 March 2018 2,621 3 View
I started off by creating a stable knockdown model using a control shRNA and two shRNA specific for my gene-of-interest. I achieved 90% knockdown after antibiotic selection, and the knockdown...
08 August 2014 7,811 7 View
I would like to use a comet assay to test the effect of my protein-of-interest on DNA damage response, double strand breaks and chemoresistance in cancer cells. There are several types of comet...
06 June 2014 7,951 3 View
I recently started using vinculin as a loading control for high molecular weight proteins. Strangely, vinculin showed up evenly despite a 2-fold difference in loading. I wonder what is happening...
02 February 2014 8,780 3 View
My proteins of interests are ~350-500kDa. The usual loading controls have much lower molecular weights. I run 6% (non-gradient) gels. Are there any suitable loading controls out there?
02 February 2014 1,472 5 View
I lysed my cells in 1x Laemmli buffer for 2 hours by mistake (the usual time period is 15mins). Would this mistake degrade my samples? I'm looking for phosphoproteins.
11 November 2013 4,988 3 View
A protein of interest has an undetectable mRNA expression level in my cell line. I knockdown using siRNA (and overexpressed, but that's a different story) and surprisingly was able to detect the...
07 July 2013 1,016 8 View
